cell lines hek293 atcc crl Search Results


hek293  (ATCC)
99
ATCC hek293
(A) Cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected for 2, 12, 24, and 48 hpi. were resolved on 4-20% SDS-PAGE. Western blots for HMGB1 in the nuclear (Nuc), cytoplasmic (Cyt), and supernatant (Sup) extracts with TBP (nuclear) β-actin (cytoplasmic) as loading controls in cell types THE, PHCE, HCF, A549, and <t>HEK293</t> (left to right). (B) Densitometric analysis of HMGB1 band intensity of cytoplasmic and nuclear extracts in THE, PHCE, HCF, A549, and HEK293 (left to right). p values were determined by t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ( n=3 ). (C) Quantification of extracellular HMGB1 measured from supernatants collected at indicated time points pi in THE and PHCE cells ( n=3 ). (D) Schematic representation (created using Biorender.com ) for HAdV-D37 induced translocation of HMGB1 from the cell nucleus to the cytoplasm and then into the extracellular space.
Hek293, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
hek293 - by Bioz Stars, 2026-03
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g418  (Thermo Fisher)
96
Thermo Fisher g418
(A) Cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected for 2, 12, 24, and 48 hpi. were resolved on 4-20% SDS-PAGE. Western blots for HMGB1 in the nuclear (Nuc), cytoplasmic (Cyt), and supernatant (Sup) extracts with TBP (nuclear) β-actin (cytoplasmic) as loading controls in cell types THE, PHCE, HCF, A549, and <t>HEK293</t> (left to right). (B) Densitometric analysis of HMGB1 band intensity of cytoplasmic and nuclear extracts in THE, PHCE, HCF, A549, and HEK293 (left to right). p values were determined by t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ( n=3 ). (C) Quantification of extracellular HMGB1 measured from supernatants collected at indicated time points pi in THE and PHCE cells ( n=3 ). (D) Schematic representation (created using Biorender.com ) for HAdV-D37 induced translocation of HMGB1 from the cell nucleus to the cytoplasm and then into the extracellular space.
G418, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sars-cov-2 spike glycoprotein (s2), sheep fc-tag (hek293)  (Native Antigen Inc)
92
Native Antigen Inc sars-cov-2 spike glycoprotein (s2), sheep fc-tag (hek293)
(A) Cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected for 2, 12, 24, and 48 hpi. were resolved on 4-20% SDS-PAGE. Western blots for HMGB1 in the nuclear (Nuc), cytoplasmic (Cyt), and supernatant (Sup) extracts with TBP (nuclear) β-actin (cytoplasmic) as loading controls in cell types THE, PHCE, HCF, A549, and <t>HEK293</t> (left to right). (B) Densitometric analysis of HMGB1 band intensity of cytoplasmic and nuclear extracts in THE, PHCE, HCF, A549, and HEK293 (left to right). p values were determined by t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ( n=3 ). (C) Quantification of extracellular HMGB1 measured from supernatants collected at indicated time points pi in THE and PHCE cells ( n=3 ). (D) Schematic representation (created using Biorender.com ) for HAdV-D37 induced translocation of HMGB1 from the cell nucleus to the cytoplasm and then into the extracellular space.
Sars Cov 2 Spike Glycoprotein (S2), Sheep Fc Tag (Hek293), supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sars coronavirus spike glycoprotein (s1), his-tag (hek293)  (Native Antigen Inc)
91
Native Antigen Inc sars coronavirus spike glycoprotein (s1), his-tag (hek293)
(A) Cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected for 2, 12, 24, and 48 hpi. were resolved on 4-20% SDS-PAGE. Western blots for HMGB1 in the nuclear (Nuc), cytoplasmic (Cyt), and supernatant (Sup) extracts with TBP (nuclear) β-actin (cytoplasmic) as loading controls in cell types THE, PHCE, HCF, A549, and <t>HEK293</t> (left to right). (B) Densitometric analysis of HMGB1 band intensity of cytoplasmic and nuclear extracts in THE, PHCE, HCF, A549, and HEK293 (left to right). p values were determined by t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ( n=3 ). (C) Quantification of extracellular HMGB1 measured from supernatants collected at indicated time points pi in THE and PHCE cells ( n=3 ). (D) Schematic representation (created using Biorender.com ) for HAdV-D37 induced translocation of HMGB1 from the cell nucleus to the cytoplasm and then into the extracellular space.
Sars Coronavirus Spike Glycoprotein (S1), His Tag (Hek293), supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human coronavirus nl63 spike glycoprotein (s1), his-tag (hek293)  (Native Antigen Inc)
92
Native Antigen Inc human coronavirus nl63 spike glycoprotein (s1), his-tag (hek293)
(A) Cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected for 2, 12, 24, and 48 hpi. were resolved on 4-20% SDS-PAGE. Western blots for HMGB1 in the nuclear (Nuc), cytoplasmic (Cyt), and supernatant (Sup) extracts with TBP (nuclear) β-actin (cytoplasmic) as loading controls in cell types THE, PHCE, HCF, A549, and <t>HEK293</t> (left to right). (B) Densitometric analysis of HMGB1 band intensity of cytoplasmic and nuclear extracts in THE, PHCE, HCF, A549, and HEK293 (left to right). p values were determined by t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ( n=3 ). (C) Quantification of extracellular HMGB1 measured from supernatants collected at indicated time points pi in THE and PHCE cells ( n=3 ). (D) Schematic representation (created using Biorender.com ) for HAdV-D37 induced translocation of HMGB1 from the cell nucleus to the cytoplasm and then into the extracellular space.
Human Coronavirus Nl63 Spike Glycoprotein (S1), His Tag (Hek293), supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human coronavirus hku1 spike glycoprotein (s1), his-tag (hek293)  (Native Antigen Inc)
99
Native Antigen Inc human coronavirus hku1 spike glycoprotein (s1), his-tag (hek293)
(A) Cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected for 2, 12, 24, and 48 hpi. were resolved on 4-20% SDS-PAGE. Western blots for HMGB1 in the nuclear (Nuc), cytoplasmic (Cyt), and supernatant (Sup) extracts with TBP (nuclear) β-actin (cytoplasmic) as loading controls in cell types THE, PHCE, HCF, A549, and <t>HEK293</t> (left to right). (B) Densitometric analysis of HMGB1 band intensity of cytoplasmic and nuclear extracts in THE, PHCE, HCF, A549, and HEK293 (left to right). p values were determined by t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ( n=3 ). (C) Quantification of extracellular HMGB1 measured from supernatants collected at indicated time points pi in THE and PHCE cells ( n=3 ). (D) Schematic representation (created using Biorender.com ) for HAdV-D37 induced translocation of HMGB1 from the cell nucleus to the cytoplasm and then into the extracellular space.
Human Coronavirus Hku1 Spike Glycoprotein (S1), His Tag (Hek293), supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sars-cov-2 (b.1.351/501.v2) stabilized spike glycoprotein (full-length), his-strep-tag (hek293)  (Native Antigen Inc)
93
Native Antigen Inc sars-cov-2 (b.1.351/501.v2) stabilized spike glycoprotein (full-length), his-strep-tag (hek293)
(A) Cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected for 2, 12, 24, and 48 hpi. were resolved on 4-20% SDS-PAGE. Western blots for HMGB1 in the nuclear (Nuc), cytoplasmic (Cyt), and supernatant (Sup) extracts with TBP (nuclear) β-actin (cytoplasmic) as loading controls in cell types THE, PHCE, HCF, A549, and <t>HEK293</t> (left to right). (B) Densitometric analysis of HMGB1 band intensity of cytoplasmic and nuclear extracts in THE, PHCE, HCF, A549, and HEK293 (left to right). p values were determined by t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ( n=3 ). (C) Quantification of extracellular HMGB1 measured from supernatants collected at indicated time points pi in THE and PHCE cells ( n=3 ). (D) Schematic representation (created using Biorender.com ) for HAdV-D37 induced translocation of HMGB1 from the cell nucleus to the cytoplasm and then into the extracellular space.
Sars Cov 2 (B.1.351/501.V2) Stabilized Spike Glycoprotein (Full Length), His Strep Tag (Hek293), supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cd147 (bsg) recombinant protein, his-tag (hek293)  (Native Antigen Inc)
94
Native Antigen Inc human cd147 (bsg) recombinant protein, his-tag (hek293)
(A) Cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected for 2, 12, 24, and 48 hpi. were resolved on 4-20% SDS-PAGE. Western blots for HMGB1 in the nuclear (Nuc), cytoplasmic (Cyt), and supernatant (Sup) extracts with TBP (nuclear) β-actin (cytoplasmic) as loading controls in cell types THE, PHCE, HCF, A549, and <t>HEK293</t> (left to right). (B) Densitometric analysis of HMGB1 band intensity of cytoplasmic and nuclear extracts in THE, PHCE, HCF, A549, and HEK293 (left to right). p values were determined by t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ( n=3 ). (C) Quantification of extracellular HMGB1 measured from supernatants collected at indicated time points pi in THE and PHCE cells ( n=3 ). (D) Schematic representation (created using Biorender.com ) for HAdV-D37 induced translocation of HMGB1 from the cell nucleus to the cytoplasm and then into the extracellular space.
Human Cd147 (Bsg) Recombinant Protein, His Tag (Hek293), supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sars-cov-2 (b.1.1.7 e484k) stabilized spike glycoprotein (full-length), his-strep-tag (hek293)  (Native Antigen Inc)
93
Native Antigen Inc sars-cov-2 (b.1.1.7 e484k) stabilized spike glycoprotein (full-length), his-strep-tag (hek293)
(A) Cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected for 2, 12, 24, and 48 hpi. were resolved on 4-20% SDS-PAGE. Western blots for HMGB1 in the nuclear (Nuc), cytoplasmic (Cyt), and supernatant (Sup) extracts with TBP (nuclear) β-actin (cytoplasmic) as loading controls in cell types THE, PHCE, HCF, A549, and <t>HEK293</t> (left to right). (B) Densitometric analysis of HMGB1 band intensity of cytoplasmic and nuclear extracts in THE, PHCE, HCF, A549, and HEK293 (left to right). p values were determined by t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ( n=3 ). (C) Quantification of extracellular HMGB1 measured from supernatants collected at indicated time points pi in THE and PHCE cells ( n=3 ). (D) Schematic representation (created using Biorender.com ) for HAdV-D37 induced translocation of HMGB1 from the cell nucleus to the cytoplasm and then into the extracellular space.
Sars Cov 2 (B.1.1.7 E484k) Stabilized Spike Glycoprotein (Full Length), His Strep Tag (Hek293), supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sars-cov-2 (b.1.351) stabilized spike glycoprotein (trimeric), his-strep-tag (hek293)  (Native Antigen Inc)
89
Native Antigen Inc sars-cov-2 (b.1.351) stabilized spike glycoprotein (trimeric), his-strep-tag (hek293)
(A) Cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected for 2, 12, 24, and 48 hpi. were resolved on 4-20% SDS-PAGE. Western blots for HMGB1 in the nuclear (Nuc), cytoplasmic (Cyt), and supernatant (Sup) extracts with TBP (nuclear) β-actin (cytoplasmic) as loading controls in cell types THE, PHCE, HCF, A549, and <t>HEK293</t> (left to right). (B) Densitometric analysis of HMGB1 band intensity of cytoplasmic and nuclear extracts in THE, PHCE, HCF, A549, and HEK293 (left to right). p values were determined by t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ( n=3 ). (C) Quantification of extracellular HMGB1 measured from supernatants collected at indicated time points pi in THE and PHCE cells ( n=3 ). (D) Schematic representation (created using Biorender.com ) for HAdV-D37 induced translocation of HMGB1 from the cell nucleus to the cytoplasm and then into the extracellular space.
Sars Cov 2 (B.1.351) Stabilized Spike Glycoprotein (Trimeric), His Strep Tag (Hek293), supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adiponectin human hek293  (BioVendor Instruments)
94
BioVendor Instruments adiponectin human hek293
(A) Cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected for 2, 12, 24, and 48 hpi. were resolved on 4-20% SDS-PAGE. Western blots for HMGB1 in the nuclear (Nuc), cytoplasmic (Cyt), and supernatant (Sup) extracts with TBP (nuclear) β-actin (cytoplasmic) as loading controls in cell types THE, PHCE, HCF, A549, and <t>HEK293</t> (left to right). (B) Densitometric analysis of HMGB1 band intensity of cytoplasmic and nuclear extracts in THE, PHCE, HCF, A549, and HEK293 (left to right). p values were determined by t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ( n=3 ). (C) Quantification of extracellular HMGB1 measured from supernatants collected at indicated time points pi in THE and PHCE cells ( n=3 ). (D) Schematic representation (created using Biorender.com ) for HAdV-D37 induced translocation of HMGB1 from the cell nucleus to the cytoplasm and then into the extracellular space.
Adiponectin Human Hek293, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hek293 cells  (CLS Cell Lines Service GmbH)
95
CLS Cell Lines Service GmbH hek293 cells
(A) Cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected for 2, 12, 24, and 48 hpi. were resolved on 4-20% SDS-PAGE. Western blots for HMGB1 in the nuclear (Nuc), cytoplasmic (Cyt), and supernatant (Sup) extracts with TBP (nuclear) β-actin (cytoplasmic) as loading controls in cell types THE, PHCE, HCF, A549, and <t>HEK293</t> (left to right). (B) Densitometric analysis of HMGB1 band intensity of cytoplasmic and nuclear extracts in THE, PHCE, HCF, A549, and HEK293 (left to right). p values were determined by t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ( n=3 ). (C) Quantification of extracellular HMGB1 measured from supernatants collected at indicated time points pi in THE and PHCE cells ( n=3 ). (D) Schematic representation (created using Biorender.com ) for HAdV-D37 induced translocation of HMGB1 from the cell nucleus to the cytoplasm and then into the extracellular space.
Hek293 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected for 2, 12, 24, and 48 hpi. were resolved on 4-20% SDS-PAGE. Western blots for HMGB1 in the nuclear (Nuc), cytoplasmic (Cyt), and supernatant (Sup) extracts with TBP (nuclear) β-actin (cytoplasmic) as loading controls in cell types THE, PHCE, HCF, A549, and HEK293 (left to right). (B) Densitometric analysis of HMGB1 band intensity of cytoplasmic and nuclear extracts in THE, PHCE, HCF, A549, and HEK293 (left to right). p values were determined by t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ( n=3 ). (C) Quantification of extracellular HMGB1 measured from supernatants collected at indicated time points pi in THE and PHCE cells ( n=3 ). (D) Schematic representation (created using Biorender.com ) for HAdV-D37 induced translocation of HMGB1 from the cell nucleus to the cytoplasm and then into the extracellular space.

Journal: bioRxiv

Article Title: Virus and Cell Specific HMGB1 Secretion and Subepithelial Infiltrate Formation in Adenovirus Keratitis

doi: 10.1101/2025.01.07.631509

Figure Lengend Snippet: (A) Cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected for 2, 12, 24, and 48 hpi. were resolved on 4-20% SDS-PAGE. Western blots for HMGB1 in the nuclear (Nuc), cytoplasmic (Cyt), and supernatant (Sup) extracts with TBP (nuclear) β-actin (cytoplasmic) as loading controls in cell types THE, PHCE, HCF, A549, and HEK293 (left to right). (B) Densitometric analysis of HMGB1 band intensity of cytoplasmic and nuclear extracts in THE, PHCE, HCF, A549, and HEK293 (left to right). p values were determined by t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ( n=3 ). (C) Quantification of extracellular HMGB1 measured from supernatants collected at indicated time points pi in THE and PHCE cells ( n=3 ). (D) Schematic representation (created using Biorender.com ) for HAdV-D37 induced translocation of HMGB1 from the cell nucleus to the cytoplasm and then into the extracellular space.

Article Snippet: A549 (CCL-185), a human lung carcinoma cell line, and HEK293 (CRL-1573), a human embryonic kidney cell line, were purchased from American Type Culture Collection (ATCC).

Techniques: Infection, SDS Page, Western Blot, Translocation Assay

(A) Western blot analysis for HMGB1 expression along with β-actin for load control from whole cell lysates of uninfected (M) or HAdV-D37-infected (V) THE cells for 2, 12, 24, and 48 hpi. qRT-PCR analysis of HMGB1 gene expression for mock and virus infected cells at the same times pi is shown as bar graphs below the Western blot. (B) Bar graph for qRT-PCR of the viral early gene E1A expression, a surrogate marker for viral entry, and normalized to human ACTG gene for quantification. (C) Viral late protein pIIIa expression in cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected THE, PHCE, HCF, A549 and HEK293 for 2, 12, 24, and 48 hpi show successful infection of all cell types.

Journal: bioRxiv

Article Title: Virus and Cell Specific HMGB1 Secretion and Subepithelial Infiltrate Formation in Adenovirus Keratitis

doi: 10.1101/2025.01.07.631509

Figure Lengend Snippet: (A) Western blot analysis for HMGB1 expression along with β-actin for load control from whole cell lysates of uninfected (M) or HAdV-D37-infected (V) THE cells for 2, 12, 24, and 48 hpi. qRT-PCR analysis of HMGB1 gene expression for mock and virus infected cells at the same times pi is shown as bar graphs below the Western blot. (B) Bar graph for qRT-PCR of the viral early gene E1A expression, a surrogate marker for viral entry, and normalized to human ACTG gene for quantification. (C) Viral late protein pIIIa expression in cytoplasmic and nuclear extracts prepared from uninfected or HAdV-D37-infected THE, PHCE, HCF, A549 and HEK293 for 2, 12, 24, and 48 hpi show successful infection of all cell types.

Article Snippet: A549 (CCL-185), a human lung carcinoma cell line, and HEK293 (CRL-1573), a human embryonic kidney cell line, were purchased from American Type Culture Collection (ATCC).

Techniques: Western Blot, Expressing, Control, Infection, Quantitative RT-PCR, Gene Expression, Virus, Marker